Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121)
CAT#: HPAB-0273-YC
The antibodies bind to the second extracellular loop of the human β1-adrenoreceptor (β1-AR-ECII). The antibodies are particularly useful in determination of auto-anti-β1-AR antibodies in an in vitro cell based assay system in order to characterize and to identify auto-antibodies directed against the β1-AR-ECII in a biological sample.
Gene Expression
Tested Data
Normal Tissue
Figure 1 Cerebral cortex
Glial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67972/149850_B_7_5.jpg
Normal Tissue
Figure 2 Kidney
Proximal tubules (cell body)
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67972/149850_A_8_5.jpg
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SDS-PAGE
Figure 1 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in SDS-PAGE
SDS-PAGE analysis of HPAB-0273-YC in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in SEC-HPLC
The purity of this antibody is> 95% as determined by SEC-HPLC.
WB
Figure 3 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in WB
Western blot analysis of HPAB-0273-YC was performed by loading ADRB1 peptide (HWWRAESDEARRCYNDPKCCDFVTNR-BSA).
HPAB-0273-YC incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat anti-Mouse IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
ELISA
Figure 4 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in ELISA
ELISA analysis of HPAB-0273-YC was performed by coating with ADRB1 peptide (HWWRAESDEARRCYNDPKCCDFVTNR-BSA).
The secondary antibody: HRP-Goat anti-Mouse IgG
DB
Figure 5 Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121) (HPAB-0273-YC) in DB
Dot Blot analysis of HPAB-0273-YC was performed by coating with ADRB1 peptide (HWWRAESDEARRCYNDPKCCDFVTNR-BSA).
The secondary antibody: HRP-Goat anti-Mouse IgG
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Specifications
- Immunogen
- GST fusion protein linked with a 31-meric peptide (GST-131-AR-ECII)
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human ADRB1
- Species Reactivity
- Human
- Clone
- DSM ACC3121
- Applications
- ELISA
- Related Disease
- DCM, ICM
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Great for Tissue Studies
worked incredibly well for tissue research, offering a precise and transparent staining that simplified analysis.
Trusted by Our Lab
Our lab uses this antibody because it consistently yields high-quality data and is dependable.
Impressive Antibody
A remarkable antibody that functions effectively in a range of experimental setups. Will keep using it.
Q&As
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What steps should I take if my antibody isn't producing expected results?
A: Review the protocol, ensure proper sample preparation, verify antibody concentration and incubation times, and include appropriate controls.
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What is the best way to ensure the antibody's stability over time?
A: Store according to manufacturer's recommendations, aliquot to avoid repeated freeze-thaw cycles, and add stabilizers if suggested.
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How do I select an appropriate secondary antibody for my experiment?
A: Choose based on species reactivity and whether it fits your detection system (e.g., fluorescence, chemiluminescence).
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0273-YC, RRID: AB_3111378)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone DSM ACC3121"
- CAT
- Product Name
See other products for "ADRB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-114-VHH | Recombinant Anti-Human ADRB1 VHH Single Domain Antibody | ICC, ChiP, FC, FA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1381z | Rabbit Anti-ADRB1 Recombinant Antibody (MOB-1381z) | WB | Rabbit IgG |
| HPAB-0274-YC | Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3176) | ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1381z-F(E) | Recombinant Anti-Human ADRB1 Antibody Fab Fragment | ELISA, Nert, FuncS | Fab |
| HPAB-0273-YC-F(E) | Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121); Fab Fragment | ELISA | Mouse Fab |
| HPAB-0274-YC-F(E) | Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3176); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1381z-S(P) | Recombinant Anti-Human ADRB1 Antibody scFv Fragment | WB, IHC, FuncS | scFv |
| HPAB-0273-YC-S(P) | Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3121); scFv Fragment | ELISA | Mouse scFv |
| HPAB-0274-YC-S(P) | Mouse Anti-ADRB1 Recombinant Antibody (clone DSM ACC3176); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1250CL | Anti-Human ADRB1 Recombinant Antibody (45084) | ELISA | |
| TAB-1250CL-S(P) | Anti-Human ADRB1 Recombinant Antibody scFv Fragment (45084) | ELISA | |
| TAB-1250CL-F(E) | Anti-Human ADRB1 Recombinant Antibody Fab Fragment (45084) | ELISA |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC592 | Recombinant Anti-ADRB1 Vesicular Antibody, EV Displayed (VS-0425-YC592) | ELISA, FC, Cell-uptake |
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